The man was unloading stock from a truck at Strathalbyn around 6pm on Thursday when he was attacked, AAP reports.
“We believe that he was in the back of the truck unloading the bull at the time,” a police spokeswoman told ABC 891.
“There was more than one buffalo in the back that the man was trying to unload.”
He was treated at the scene by paramedics before being flown to Flinders Medical Centre but died a short time later. Safework SA is investigating the matter.